Bulk methylene blue powder

What is bulk methylene blue powder?

Bulk methylene blue powder is a phenothiazine salt, dark green bronze luster crystal or powder, soluble in water and ethanol, insoluble in ether. It is relatively stable in air and can form complex salts with most inorganic salts. The aqueous solution is alkaline. Low toxicity, avoid contact with skin and eyes. Adding dilute sulfuric acid to its solution will cause the solution to fade. If ammonia water is added quickly or it is exposed to the air, it can be restored.

Staining principle:

Methylene blue staining is a commonly used histological staining method, often used to stain cell nuclei or intracellular structures. It is an alkaline dye that can bind to anions in DNA molecules to form a dark blue precipitate.
Its principle mainly includes two steps: fixation and coloring. Fixation refers to fixing the cell tissue to be stained so that it maintains its original structure and morphology. Commonly used fixatives include formalin, acetaldehyde, etc. During the fixation process, the DNA in the cell tissue will undergo alkaline hydrolysis, and the phosphate groups in the DNA molecules will release H+, thereby generating negative charges.
During the coloring process, the fixed tissue is placed in a staining solution containing methylene blue dye. The dye contains cationic groups that can bind to negative charges in DNA to form a complex of methylene blue and DNA. This combination is carried out in the form of non-covalent bonds such as electrostatic action or hydrogen bonds. When the two are combined, a dark blue precipitate will be formed. This staining method has a good affinity for DNA and can clearly show the structure of the cell nucleus or the cell.
The advantages of staining are simple operation, obvious staining effect, and can clearly show the morphology and structure of the cell nucleus. However, due to its alkalinity, the cells may be unevenly stained after staining, affecting the accuracy of the staining results. In addition, during the staining process, it is also necessary to control the staining time and temperature to avoid over-staining or under-staining. After staining, washing and sealing steps are required to protect and fix the staining results.

Application:

Methylene blue is widely used in chemical indicators, dyes, biological stains and drugs. Especially in the dyeing industry, it is used to make inks, etc., and the amount is large. In the following fields, methylene blue is also widely used.
1. Industrial field
Belonging to dye compounds, industrial-grade methylene blue is often used for dyeing cotton, silk, paper, etc., and can also be used in bamboo and wood coloring and the manufacture of inks, lakes, etc. In addition, it is also often used as bacterial stains, indicators, etc.
2. Medical field
It has been used in the medical field a long time ago. Due to its redox properties, it can be used to relieve poisoning caused by cyanide, nitrite, aniline, acetanilide, or methemoglobinemia caused by drugs such as sulfonamides. In recent years, scholars have also studied its application in various bacterial and viral infections and central nervous system diseases such as depression, schizophrenia, Alzheimer's disease, etc.
3. Aquaculture field
It can be used as a disinfectant in aquaculture. The principle is that the ionic compounds formed in the aqueous solution can compete with the microbial enzyme system for hydrogen ions, inactivate the enzyme, and then cause the microorganisms to lose their ability to survive; it also has a certain effect in the treatment of fish diseases such as Ichthyophthirius, trichomoniasis, red mouth disease, Saprolegniasis, and Penaeus vannamei larvae slime disease. It can also be used as an antifungal drug to reduce the mortality rate of fish during transportation.
4. Skin care field
Studies have shown that it also has certain potential in skin care. It can scavenge free radicals, stimulate cell proliferation in young and old dermal fibroblasts, and thus has certain effects on improving skin vitality, promoting skin elasticity and collagen synthesis, and protecting the skin matrix through the degradation of matrix metalloproteinases. Therefore, it can become a promising agent in anti-aging cosmetics.
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